-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
Originator-Key-Asymmetric:
 MFgwCgYEVQgBAQICAf8DSgAwRwJAW2sNKK9AVtBzYZmr6aGjlWyK3XmZv3dTINen
 TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB
MIC-Info: RSA-MD5,RSA,
 I56amKMEd0C2mQRAimufn25EoSqebgGpgdfDBgVhGqfnWNZuOcFK3Kw5HHG2GqvY
 upl846JxGAwd7wY4oD8ThQ==

<SEC-DOCUMENT>0001011034-10-000004.txt : 20100106
<SEC-HEADER>0001011034-10-000004.hdr.sgml : 20100106
<ACCEPTANCE-DATETIME>20100106140714
ACCESSION NUMBER:		0001011034-10-000004
CONFORMED SUBMISSION TYPE:	D
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20100106
DATE AS OF CHANGE:		20100106
EFFECTIVENESS DATE:		20100106

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			VITRO DIAGNOSTICS INC
		CENTRAL INDEX KEY:			0000793171
		STANDARD INDUSTRIAL CLASSIFICATION:	BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
		IRS NUMBER:				841012042
		STATE OF INCORPORATION:			NV
		FISCAL YEAR END:			1031

	FILING VALUES:
		FORM TYPE:		D
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-19713-D
		FILM NUMBER:		10510904

	BUSINESS ADDRESS:	
		STREET 1:		4621 TECHNOLOGY DRIVE
		CITY:			GOLDEN
		STATE:			CO
		ZIP:			80403
		BUSINESS PHONE:		(720) 859-4120

	MAIL ADDRESS:	
		STREET 1:		4621 TECHNOLOGY DRIVE
		CITY:			GOLDEN
		STATE:			CO
		ZIP:			80403

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	LABTEK INC
		DATE OF NAME CHANGE:	19870217

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	IMPERIAL MANAGEMENT INC
		DATE OF NAME CHANGE:	19870201
</SEC-HEADER>
<DOCUMENT>
<TYPE>D
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0000793171</cik>
        <entityName>VITRO DIAGNOSTICS INC</entityName>
        <issuerAddress>
            <street1>4621 TECHNOLOGY DRIVE</street1>
            <city>GOLDEN</city>
            <stateOrCountry>CO</stateOrCountry>
            <zipCode>80403</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>303-999-2130</issuerPhoneNumber>
        <jurisdictionOfInc>NEVADA</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>LABTEK INC</previousName>
            <previousName>IMPERIAL MANAGEMENT INC</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>JAMES</firstName>
                <lastName>MUSICK</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>4621 TECHNOLOGY DRIVE</street1>
                <city>GOLDEN</city>
                <stateOrCountry>CO</stateOrCountry>
                <zipCode>80403</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ERIK</firstName>
                <lastName>VAN HORN</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>4621 TECHNOLOGY DRIVE</street1>
                <city>GOLDEN</city>
                <stateOrCountry>CO</stateOrCountry>
                <zipCode>80403</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>$1 - $1,000,000</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-12-18</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>87500</totalOfferingAmount>
            <totalAmountSold>87500</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>3</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>VITRO DIAGNOSTICS INC</issuerName>
                <signatureName>/s/ James Musick</signatureName>
                <nameOfSigner>JAMES MUSICK</nameOfSigner>
                <signatureTitle>President</signatureTitle>
                <signatureDate>2010-01-05</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----