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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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<SEC-DOCUMENT>0001476010-09-000002.txt : 20091218
<SEC-HEADER>0001476010-09-000002.hdr.sgml : 20091218
<ACCEPTANCE-DATETIME>20091218124915
ACCESSION NUMBER:		0001476010-09-000002
CONFORMED SUBMISSION TYPE:	D
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20091218
DATE AS OF CHANGE:		20091218
EFFECTIVENESS DATE:		20091218

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			Afferent Pharmaceuticals, Inc.
		CENTRAL INDEX KEY:			0001476010
		IRS NUMBER:				271116683
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	FILING VALUES:
		FORM TYPE:		D
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-135875
		FILM NUMBER:		091249247

	BUSINESS ADDRESS:	
		STREET 1:		C/O THIRD ROCK VENTURES, L.P.
		STREET 2:		29 NEWBURY STREET
		CITY:			BOSTON
		STATE:			MA
		ZIP:			02116
		BUSINESS PHONE:		617-585-2000

	MAIL ADDRESS:	
		STREET 1:		C/O THIRD ROCK VENTURES, L.P.
		STREET 2:		29 NEWBURY STREET
		CITY:			BOSTON
		STATE:			MA
		ZIP:			02116
</SEC-HEADER>
<DOCUMENT>
<TYPE>D
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001476010</cik>
        <entityName>Afferent Pharmaceuticals, Inc.</entityName>
        <issuerAddress>
            <street1>C/O THIRD ROCK VENTURES, L.P.</street1>
            <street2>29 NEWBURY STREET</street2>
            <city>BOSTON</city>
            <stateOrCountry>MA</stateOrCountry>
            <zipCode>02116</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>617-585-2000</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2009</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Kevin</firstName>
                <lastName>Starr</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Third Rock Ventures, L.P.</street1>
                <street2>29 Newbury Street</street2>
                <city>Boston</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02116</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>President</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>David</firstName>
                <lastName>Neafus</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Third Rock Ventures, L.P.</street1>
                <street2>29 Newbury Street</street2>
                <city>Boston</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02116</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Financial Officer &amp; Treasurer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Mitchell</firstName>
                <middleName>S.</middleName>
                <lastName>Bloom</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Goodwin Procter LLP</street1>
                <street2>Exchange Place</street2>
                <city>Boston</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02109</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Secretary</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Jesse</firstName>
                <lastName>Treu</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Domain Associates, LLC</street1>
                <street2>One Palmer Square, Suite 515</street2>
                <city>Princeton</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08542</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Liam</firstName>
                <lastName>Ratcliffe</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>New Leaf Venture Partners</street1>
                <street2>7 Times Square, Suite 1603</street2>
                <city>New York</city>
                <stateOrCountry>NY</stateOrCountry>
                <zipCode>10036</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Arthur</firstName>
                <lastName>Pappas</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Pappas Ventures</street1>
                <street2>2520 Meridian Parkway, Suite 400</street2>
                <city>Durham</city>
                <stateOrCountry>NC</stateOrCountry>
                <zipCode>27713</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other Health Care</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-12-09</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>23000000</totalOfferingAmount>
            <totalAmountSold>3000000</totalAmountSold>
            <totalRemaining>20000000</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>6</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
                <isEstimate>true</isEstimate>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Afferent Pharmaceuticals, Inc.</issuerName>
                <signatureName>David Neafus</signatureName>
                <nameOfSigner>David Neafus</nameOfSigner>
                <signatureTitle>Chief Financial Officer, Treasurer &amp; Assistant Secretary</signatureTitle>
                <signatureDate>2009-12-09</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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