-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
 BZhzYAlFBs39GwykdvMZpHaiX0MHmZUR2dAusJdQ8M4FUHe+/z/e6NJHvO46G5Vu
 CPfj2ZaUU2XMwFD2nOX+pQ==

<SEC-DOCUMENT>0001424288-10-000002.txt : 20100518
<SEC-HEADER>0001424288-10-000002.hdr.sgml : 20100518
<ACCEPTANCE-DATETIME>20100518124136
ACCESSION NUMBER:		0001424288-10-000002
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20100518
DATE AS OF CHANGE:		20100518
EFFECTIVENESS DATE:		20100518

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			AFFINITY BIOSENSORS LLC
		CENTRAL INDEX KEY:			0001424288
		IRS NUMBER:				000000000
		STATE OF INCORPORATION:			CA

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-113512
		FILM NUMBER:		10841722

	BUSINESS ADDRESS:	
		STREET 1:		75D ROBIN HILL ROAD
		CITY:			GOLETA
		STATE:			CA
		ZIP:			93117
		BUSINESS PHONE:		805-455-0181

	MAIL ADDRESS:	
		STREET 1:		75D ROBIN HILL ROAD
		CITY:			GOLETA
		STATE:			CA
		ZIP:			93117
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0704</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001424288</cik>
        <entityName>AFFINITY BIOSENSORS LLC</entityName>
        <issuerAddress>
            <street1>75D ROBIN HILL ROAD</street1>
            <city>GOLETA</city>
            <stateOrCountry>CA</stateOrCountry>
            <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
            <zipCode>93117</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>805-455-0181</issuerPhoneNumber>
        <jurisdictionOfInc>CALIFORNIA</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Liability Company</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2006</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Kenneth</firstName>
                <lastName>Babcock</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>75 D Robin Hill Road</street1>
                <city>Goleta</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>93117</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Manager and President</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Not Applicable</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>9999999997-08-042430</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2008-09-30</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isOtherType>true</isOtherType>
            <descriptionOfOtherType>Series A Preferred Membership Units</descriptionOfOtherType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>1100000</totalOfferingAmount>
            <totalAmountSold>890000</totalAmountSold>
            <totalRemaining>210000</totalRemaining>
            <clarificationOfResponse>The Company extended its Series A Preferred offering and shall use the proceeds for working capital.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>11</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>AFFINITY BIOSENSORS LLC</issuerName>
                <signatureName>/s/ Kenneth Babcock</signatureName>
                <nameOfSigner>Kenneth Babcock</nameOfSigner>
                <signatureTitle>President</signatureTitle>
                <signatureDate>2010-05-18</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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