-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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 UqQvdUzQNQN0NH+fWOBAnA==

<SEC-DOCUMENT>0001250853-09-000030.txt : 20090413
<SEC-HEADER>0001250853-09-000030.hdr.sgml : 20090413
<ACCEPTANCE-DATETIME>20090413145905
ACCESSION NUMBER:		0001250853-09-000030
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20090413
DATE AS OF CHANGE:		20090413
EFFECTIVENESS DATE:		20090413

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			LIXTE BIOTECHNOLOGY HOLDINGS, INC.
		CENTRAL INDEX KEY:			0001335105
		STANDARD INDUSTRIAL CLASSIFICATION:	PHARMACEUTICAL PREPARATIONS [2834]
		IRS NUMBER:				202903526
		STATE OF INCORPORATION:			DE

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-94096
		FILM NUMBER:		09746477

	BUSINESS ADDRESS:	
		STREET 1:		248 ROUTE 25A
		STREET 2:		NO. 2
		CITY:			EAST SETAUKET
		STATE:			NY
		ZIP:			11733
		BUSINESS PHONE:		310 203 2902

	MAIL ADDRESS:	
		STREET 1:		248 ROUTE 25A
		STREET 2:		NO. 2
		CITY:			EAST SETAUKET
		STATE:			NY
		ZIP:			11733

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	SRKP 7 INC
		DATE OF NAME CHANGE:	20050803
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0301</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001335105</cik>
        <entityName>LIXTE BIOTECHNOLOGY HOLDINGS, INC.</entityName>
        <issuerAddress>
            <street1>248 ROUTE 25A NO. 2</street1>
            <street2></street2>
            <city>EAST SETAUKET</city>
            <stateOrCountry>NY</stateOrCountry>
            <zipCode>11733</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>631-942-7959</issuerPhoneNumber>
        <issuerPreviousNameList>
            <previousName>SRKP 7 INC</previousName>
        </issuerPreviousNameList>
        <jurisdictionOfInc>DE</jurisdictionOfInc>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2005</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>John</firstName>
                <middleName>S.</middleName>
                <lastName>Kovach</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>248 Route 25A No. 2</street1>
                <street2></street2>
                <city>East Setauket</city>
                <stateOrCountry>NY</stateOrCountry>
                <zipCode>11733</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Stephen</firstName>
                <middleName>K.</middleName>
                <lastName>Carter</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>248 Route 25A No. 2</street1>
                <street2></street2>
                <city>East Setauket</city>
                <stateOrCountry>NY</stateOrCountry>
                <zipCode>11733</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Philip</firstName>
                <middleName>F.</middleName>
                <lastName>Palmedo</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>248 Route 25A No. 2</street1>
                <street2></street2>
                <city>East Setauket</city>
                <stateOrCountry>NY</stateOrCountry>
                <zipCode>11733</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Mel</firstName>
                <middleName></middleName>
                <lastName>Sorenson</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>248 Route 25A No. 2</street1>
                <street2></street2>
                <city>East Setauket</city>
                <stateOrCountry>NY</stateOrCountry>
                <zipCode>11733</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>No Revenues</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>9999999997-09-004895</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-02-10</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>None</recipientName>
                <recipientCRDNumber>None</recipientCRDNumber>
                <associatedBDName>WestPark Capital, Inc.</associatedBDName>
                <associatedBDCRDNumber>39914</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1900 Avenue of the Stars, Suite 310</street1>
                    <street2></street2>
                    <city>Los Angeles</city>
                    <stateOrCountry>CA</stateOrCountry>
                    <zipCode>90067</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>AR</state>
                    <state>CA</state>
                    <state>DC</state>
                    <state>FL</state>
                    <state>GA</state>
                    <state>MA</state>
                    <state>MI</state>
                    <state>MS</state>
                    <state>NH</state>
                    <state>NJ</state>
                    <state>NY</state>
                    <state>OH</state>
                    <state>PA</state>
                    <state>TX</state>
                    <state>UT</state>
                    <state>WI</state>
                </statesOfSolicitationList>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>2000000</totalOfferingAmount>
            <totalAmountSold>710000</totalAmountSold>
            <totalRemaining>1290000</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>28</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>71000</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>28400</dollarAmount>
            </findersFees>
            <clarificationOfResponse>*Plus 5 year warrants to purchase 142,000 shares of Issuer's Common Stock and 5 year warrants to purchase an additional 28,400 shares.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>LIXTE BIOTECHNOLOGY HOLDINGS, INC.</issuerName>
                <signatureName>/s/ John S. Kovach</signatureName>
                <nameOfSigner>John S. Kovach</nameOfSigner>
                <signatureTitle>Chief Executive Officer</signatureTitle>
                <signatureDate>2009-04-10</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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