-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
Originator-Key-Asymmetric:
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 TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB
MIC-Info: RSA-MD5,RSA,
 TwfBFEpYfT7vrzgmoxmTk3YUgevWO6vrQzvtbY31AxXzRE7hH3ohsP+864dM2N7r
 QmNSol2uiBcYvjhEdOMBCA==

<SEC-DOCUMENT>0001186197-09-000004.txt : 20091013
<SEC-HEADER>0001186197-09-000004.hdr.sgml : 20091012
<ACCEPTANCE-DATETIME>20091009183814
ACCESSION NUMBER:		0001186197-09-000004
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20091013
DATE AS OF CHANGE:		20091009
EFFECTIVENESS DATE:		20091013

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			TRINITY BIOSYSTEMS INC
		CENTRAL INDEX KEY:			0001186197
		IRS NUMBER:				000000000
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-47911
		FILM NUMBER:		091115002

	BUSINESS ADDRESS:	
		STREET 1:		1490 O'BRIEN AVENUE
		CITY:			MENLO PARK
		STATE:			CA
		ZIP:			94025
		BUSINESS PHONE:		650-566-0780

	MAIL ADDRESS:	
		STREET 1:		1490 O'BRIEN AVENUE
		CITY:			MENLO PARK
		STATE:			CA
		ZIP:			94025
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001186197</cik>
        <entityName>TRINITY BIOSYSTEMS INC</entityName>
        <issuerAddress>
            <street1>1490 O'BRIEN AVENUE</street1>
            <city>MENLO PARK</city>
            <stateOrCountry>CA</stateOrCountry>
            <zipCode>94025</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>650-566-0780</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Amin</firstName>
                <lastName>Khan</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Trinity Biosystems, Inc.</street1>
                <street2>1490 O'Brien Avenue</street2>
                <city>Menlo Park</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94025</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Michael</firstName>
                <middleName>C.</middleName>
                <lastName>Diem</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o S.R. One Limited</street1>
                <street2>161 Washington St., Suite 500</street2>
                <city>Conshohocken</city>
                <stateOrCountry>PA</stateOrCountry>
                <zipCode>19428</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Rene</firstName>
                <lastName>Kuijten</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Johannes Vermeerplein 9</street1>
                <city>Amsterdam</city>
                <stateOrCountry>P7</stateOrCountry>
                <zipCode>1071 DV</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Peter</firstName>
                <middleName>C.</middleName>
                <lastName>McWilliams</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Sanderling Ventures</street1>
                <street2>400 S. El Camino Real, Suite 1200</street2>
                <city>San Mateo</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94402</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>S.</firstName>
                <middleName>Edward</middleName>
                <lastName>Torres</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Lilly Ventures</street1>
                <street2>Lilly Corporate Center (DC 1032)</street2>
                <city>Indianapolis</city>
                <stateOrCountry>IN</stateOrCountry>
                <zipCode>46285</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Helen</firstName>
                <lastName>Kim</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Trinity Biosystems, Inc.</street1>
                <street2>1490 O'Brien Avenue</street2>
                <city>Menlo Park</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94025</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gail</firstName>
                <lastName>Maderis</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Trinity Biosystems, Inc.</street1>
                <street2>1490 O'Brien Avenue</street2>
                <city>Menlo Park</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94025</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001186197-09-000003</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-05-15</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isDebtType>true</isDebtType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
            <isOtherType>true</isOtherType>
            <descriptionOfOtherType>Sale and issuance of Secured Convertible Promissory Notes convertible into Preferred Stock, the underlying shares of Common Stock issuable upon conversion of the Preferred Stock, and the issuance of Warrants exercsiable for Common Stock</descriptionOfOtherType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>12500000</totalOfferingAmount>
            <totalAmountSold>4000000</totalAmountSold>
            <totalRemaining>8500000</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>17</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>TRINITY BIOSYSTEMS INC</issuerName>
                <signatureName>Danielle E. Reed</signatureName>
                <nameOfSigner>Danielle E. Reed</nameOfSigner>
                <signatureTitle>Secretary</signatureTitle>
                <signatureDate>2009-10-09</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----