-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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<SEC-DOCUMENT>0001019687-09-002330.txt : 20090629
<SEC-HEADER>0001019687-09-002330.hdr.sgml : 20090629
<ACCEPTANCE-DATETIME>20090626195340
ACCESSION NUMBER:		0001019687-09-002330
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20090629
DATE AS OF CHANGE:		20090626
EFFECTIVENESS DATE:		20090629

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			Cryoport, Inc.
		CENTRAL INDEX KEY:			0001124524
		STANDARD INDUSTRIAL CLASSIFICATION:	PLASTICS FOAM PRODUCTS [3086]
		IRS NUMBER:				880313393
		STATE OF INCORPORATION:			NV
		FISCAL YEAR END:			0331

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-103208
		FILM NUMBER:		09914220

	BUSINESS ADDRESS:	
		STREET 1:		20382 BARENTS SEA CIRCLE
		CITY:			LAKE FOREST
		STATE:			CA
		ZIP:			92630
		BUSINESS PHONE:		949-470-2300

	MAIL ADDRESS:	
		STREET 1:		20382 BARENTS SEA CIRCLE
		CITY:			LAKE FOREST
		STATE:			CA
		ZIP:			92630

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	CRYOPORT SYSTEMS LLC
		DATE OF NAME CHANGE:	20010614

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	CRYOPORT SYSTEMS INC
		DATE OF NAME CHANGE:	20000923
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0401</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001124524</cik>
        <entityName>Cryoport, Inc.</entityName>
        <issuerAddress>
            <street1>20382 Barents Sea Circle</street1>
            <city>Lake Forest,</city>
            <stateOrCountry>CA</stateOrCountry>
            <zipCode>92630</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>949-470-2300</issuerPhoneNumber>
        <issuerPreviousNameList>
            <previousName>G.T-5 Limited</previousName>
        </issuerPreviousNameList>
        <jurisdictionOfInc>NEVADA</jurisdictionOfInc>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Larry</firstName>
                <middleName>G.</middleName>
                <lastName>Stambaugh</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>20382 Barents Sea Circle</street1>
                <city>Lake Forest,</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>92630</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Dee</firstName>
                <middleName></middleName>
                <lastName>Kelly</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>20382 Barents Sea Circle</street1>
                <city>Lake Forest.</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>92630</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Adam</firstName>
                <middleName></middleName>
                <lastName>Michelin</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>20382 Barents Sea Circle</street1>
                <city>Lake Forest,</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>92630</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Thomas</firstName>
                <middleName></middleName>
                <lastName>Fischer</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>20382 Barents Sea Circle</street1>
                <city>Lake Forest,</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>92630</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Peter</firstName>
                <middleName></middleName>
                <lastName>Berry</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>20382 Barents Sea Circle</street1>
                <city>Lake Forest,</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>92630</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Carlton</firstName>
                <middleName></middleName>
                <lastName>Johnson</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>20382 Barents Sea Circle</street1>
                <city>Lake Forest,</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>92630</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other Technology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>$1 - $1,000,000</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001019687-09-001716</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-03-31</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isDebtType>true</isDebtType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>10000</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Emergent Financial Group</recipientName>
                <recipientCRDNumber>None</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>8000 West 78th Street, Suite 115</street1>
                    <city>Edina,</city>
                    <stateOrCountry>MN</stateOrCountry>
                    <zipCode>55439</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <state>FL</state>
                    <state>IL</state>
                    <state>IA</state>
                    <state>MN</state>
                    <state>TX</state>
                    <state>WI</state>
                </statesOfSolicitationList>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>1500000</totalOfferingAmount>
            <totalAmountSold>964500</totalAmountSold>
            <totalRemaining>535500</totalRemaining>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>25</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>90000</dollarAmount>
                <isEstimate>true</isEstimate>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>260000</dollarAmount>
                <isEstimate>true</isEstimate>
            </grossProceedsUsed>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>CryoPort, Inc.</issuerName>
                <signatureName>/s/ Larry G. Stambaugh</signatureName>
                <nameOfSigner>Larry G. Stambaugh</nameOfSigner>
                <signatureTitle>Chief Executive Officer</signatureTitle>
                <signatureDate>2009-06-26</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----