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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
 P7GLEPFU6ky6zlproGM9J3X3Kt0aT09S2dWMd2JSp6ZiUbJeDQIm+RrYZBt/OmTI
 oGRf572ul2IXPEY9wOlF5g==

<SEC-DOCUMENT>0001083672-10-000005.txt : 20100304
<SEC-HEADER>0001083672-10-000005.hdr.sgml : 20100304
<ACCEPTANCE-DATETIME>20100304170741
ACCESSION NUMBER:		0001083672-10-000005
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20100304
DATE AS OF CHANGE:		20100304
EFFECTIVENESS DATE:		20100304

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			BACTERIN INTERNATIONAL, INC.
		CENTRAL INDEX KEY:			0001083672
		STANDARD INDUSTRIAL CLASSIFICATION:	SURGICAL & MEDICAL INSTRUMENTS & APPARATUS [3841]
		IRS NUMBER:				880420501
		STATE OF INCORPORATION:			NV
		FISCAL YEAR END:			1231

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-77611
		FILM NUMBER:		10657957

	BUSINESS ADDRESS:	
		STREET 1:		600 CRUISER LANE
		CITY:			BELGRADE
		STATE:			MT
		ZIP:			59714
		BUSINESS PHONE:		(406)388-0480

	MAIL ADDRESS:	
		STREET 1:		600 CRUISER LANE
		CITY:			BELGRADE
		STATE:			MT
		ZIP:			59714

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	OIL & GAS SEEKERS INC
		DATE OF NAME CHANGE:	20000619
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001083672</cik>
        <entityName>BACTERIN INTERNATIONAL, INC.</entityName>
        <issuerAddress>
            <street1>600 CRUISER LANE</street1>
            <city>BELGRADE</city>
            <stateOrCountry>MT</stateOrCountry>
            <zipCode>59714</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>(406)388-0480</issuerPhoneNumber>
        <jurisdictionOfInc>NEVADA</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>OIL &amp; GAS SEEKERS INC</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Guy</firstName>
                <middleName>S.</middleName>
                <lastName>Cook</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Mitchell</firstName>
                <middleName>T.</middleName>
                <lastName>Godfrey</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Kent</firstName>
                <lastName>Swanson</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Steve</firstName>
                <middleName>B.</middleName>
                <lastName>Warnecke</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001083672-10-000004</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-12-02</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isDebtType>true</isDebtType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>50000</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Middlebury Securities LLC</recipientName>
                <recipientCRDNumber>122602</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1043 Sheep Farm Road</street1>
                    <city>Weybridge</city>
                    <stateOrCountry>VT</stateOrCountry>
                    <zipCode>05753</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <state>CT</state>
                    <state>FL</state>
                    <state>MN</state>
                    <state>NJ</state>
                    <state>NY</state>
                    <state>PA</state>
                </statesOfSolicitationList>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>4000000</totalOfferingAmount>
            <totalAmountSold>3000000</totalAmountSold>
            <totalRemaining>1000000</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>23</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>320000</dollarAmount>
                <isEstimate>true</isEstimate>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>Represents 8% of the maximum offering amount of $4,000,000.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>BACTERIN INTERNATIONAL, INC.</issuerName>
                <signatureName>Steve B. Warnecke</signatureName>
                <nameOfSigner>Steve B. Warnecke</nameOfSigner>
                <signatureTitle>Chief Financial Officer</signatureTitle>
                <signatureDate>2010-03-03</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----